Nanoflow LC/IMS-MS and LC/IMS-CID/MS of protein mixtures
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چکیده
منابع مشابه
Nanoflow LC/IMS-MS and LC/IMS-CID/MS of protein mixtures.
A simple ion trap/ion mobility/time-of-flight (TOF) mass spectrometer has been coupled with nanoflow liquid chromatography to examine the feasibility of analyzing mixtures of intact proteins. In this approach proteins are separated using reversed-phase chromatography. As components elute from the column, they are electrosprayed into the gas phase and separated again in a drift tube prior to bei...
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carried out in the positive mode and Capillary and Cone voltages were set at 3.3 kV and 35 V respectively. The source and desolvation temperatures were set at 150 °C and 450 °C respectively. Nitrogen was used as cone and desolvation gas wih the flow rates of 50 L/h and 450 L/h. The distinct multiple charged envelopes obtained for each protein were transformed using the maximum entropy software ...
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Nanoflow LC/MS is commonly used for protein identification and biomarker discovery, but setting up and maintaining the intricate LC plumbing can be a challenge. The Agilent HPLC-Chip represents breakthrough technology that integrates nanoflow HPLC columns, associated connections, and a nanoelectrospray emitter on a single, small, reusable microfluidic chip. The HPLC-Chip and a companion HPLCChi...
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Online nanoflow RP-RP-MS reveals dynamics of multicomponent Ku complex in response to DNA damage.
Tandem affinity purification (TAP) coupled with mass spectrometry has become the technique of choice for characterization of multicomponent protein complexes. While current TAP protocols routinely provide high yield and specificity for proteins expressed under physiologically relevant conditions, analytical figures of merit required for efficient and in-depth LC-MS analysis remain unresolved. H...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2004
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2004.06.014